Date of Award
Campus Access Thesis
Master of Science (MS)
The MAP kinase SMK1 is an important regulator of sporulation in S. cerevisiae. However, the mechanism by which Smk1 is activated and its downstream targets are not well understood. YBR063c was identified as a protein that physically interacts with Smk1 (Slubowski, Roesner, and Huang, unpublished). I characterized the phenotype of ybr063c mutants, and found that ybr063c mutants proceed through meiosis, form spores and germinate normally. I show that YBR063c expression is induced 6 hours into sporulation, and protein expression levels are unchanged in a smk1 mutant strain. I find that YBR063c localizes between the dividing nuclei during and after meiosis II, and within the spores once meiosis is complete; this localization is reminiscent of mitochondrial localization during sporulation. From this localization, I speculate that that YBR063c may play a role in mitochondrial segregation.
Funk, Alyssa, "The Role of YBR063c in the MAP Kinase Sporulation Pathway" (2015). Graduate Masters Theses. 312.