Date of Award

6-2011

Document Type

Campus Access Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Alexey Veraksa

Second Advisor

Katherine E. Gibson

Third Advisor

Linda Huang

Abstract

Notch is a transmembrane receptor that plays a crucial role in cell fate determination during development. Previous work identified Kurtz (Krz), a Drosophila homolog of mammalian β-arrestins, as a component of the Notch protein interaction network. In this study, I extended the characterization of the Notch and Krz interaction networks. For Notch, I undertook a novel in vivo approach that will allow me to follow the dynamics of Notch signaling and its interactions by expressing tagged Notch receptor at its endogenous level. Investigation of the Krz interacting proteins revealed a surprising connection with Ulp1 which is involved in regulation of SUMO conjugation.

Comments

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