Document Type
Article
Publication Date
8-24-2009
Abstract
A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control.
Recommended Citation
Lake RJ, Grimm LM, Veraksa A, Banos A, Artavanis-Tsakonas S (2009) In Vivo Analysis of the Notch Receptor S1 Cleavage. PLoS ONE 4(8): e6728. doi:10.1371/journal.pone.0006728
Publisher
Public Library of Science (PLoS)
Rights
© 2009 Lake et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Included in
Biology Commons, Cell Biology Commons, Developmental Biology Commons, Genetics and Genomics Commons
Comments
Originally published in the open access journal PLoS ONE: http://www.plosone.org.
This work was supported by grants NS2608, GM62931 and CA098402 to SA-T. LG was supported by grant NS10735, and AV was funded by the MGH fund for Medical Discovery postdoctoral fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.