Date of Award
Campus Access Thesis
Master of Science (MS)
Linda S. Huang
This work addresses the question of how morphological changes are regulated and how cell polarity and morphology are established. The work in this thesis examines the intracellular changes that occur during spore morphogenesis in the budding yeast S. cerevisiae, and focuses on the function of the gene SPO71. SPO71 is essential for spore morphogenesis and encodes a protein with two pleckstrin homology domains. SPO71 is conserved among fungi, as homologs are present in the closely related Saccharomyces species as well as more distantly related fungi. I find the prospore membrane in spo71 cells to be deficient in size compared to SPO71 prospore membranes. Structure function analysis demonstrates that the second pleckstrin homology domain is required for SPO71 function.
Baker, Crystal, "SPO71 is Required for Proper Prospore Membrane Size in Saccharomyces Cerevisiae" (2010). Graduate Masters Theses. Paper 14.