Date of Award
Campus Access Dissertation
Doctor of Philosophy (PhD)
Biology/Molecular, Cellular, and Organismal Biology
Kenneth C. Kleene
In mitosis, when chromosome attachment errors are detected, a surveillance mechanism called the spindle assembly checkpoint (SAC) delays the transition from metaphase to anaphase. When all chromosomes are accurately anchored to spindle microtubules and have migrated to the cell equator, the checkpoint is released and mitosis proceeds. Centromere-associated protein-E (CENP-E) is a motor protein from the kinesin-7 family thought to be an important contributor of the SAC. In plants, most mitotic and meiotic SAC components and processes are unknown.
Using a reverse genetics approach, I present cytological evidence that ATCENPE2 is a conserved gene of the plant mitotic checkpoint and is an important regulator of meiosis. I show that an insertion mutation, atcenpe2-1, prevents the normal alignment of chromosomes, causing a delay in mitosis. Aneuploidy is also observed in some cells, indicating an altered checkpoint function. Significantly, the atcenpe2-1 mutant shows problems in chromosome segregation during meiosis, causing gamete malformation. Together, these data describe the novel role of a previously uncharacterized kinesin in plant mitosis and meiosis. Furthermore, this study offers an important tool to study the consequences of aneuploidy at a cellular and organismal level.
Diaz, Kristophe J., "A Novel Kinesin, Arabidopsis Thaliana Centromere Associated Protein E2, is Necessary for the Progression of Mitosis and Meiosis" (2014). Graduate Doctoral Dissertations. 171.
Supplemental Movie 1 -- Described on page 74 of dissertation
Diaz -- MovieS2.mov (737 kB)
Supplemental Movie 2 -- Described on page 74 of dissertation
Diaz -- MovieS3.mov (783 kB)
Supplemental Movie 3 -- Described on page 74 of dissertation
Diaz -- MovieS4.mov (1113 kB)
Supplemental Movie 4 -- Described on page 74 of dissertation
Diaz -- MovieS5.mov (475 kB)
Supplemental Movie 5 -- Described on page 74 of dissertation
Diaz -- MovieS6.mov (904 kB)
Supplemental Movie 6 -- Described on page 74 of dissertation